Blood Draw Tube Cheat Sheet
PHLEBOTOMY SERVICES
EVACUATED BLOOD COLLECTION TUBE GUIDE.
Listed in Order of Draw for Blood Collection Tubes and Syringe Collections.
| STOPPER COLOR | SYMBOL | CONTENTS | VOL. | USES/COMMENTS |
|---|---|---|---|---|
| Blood Culture Bottles are ALWAYS drawn prior to other labs to reduce contamination. . | ||||
| Royal Blue |  | No additive (serum); special glass and stopper material | 7.0 mL | Most drug levels, toxicology screens, and trace elements |
| Red |  | No additive | 7.0 mL | Cryoglobulins and CH50 |
| Light Blue |  | 3.2% Sodium Citrate | 4.5 mL | PT, PTT, TCT, CMV buffy coat, Factor Activity Tube MUST be filled 100% - No exceptions! |
| Gold Top (Serum Separator, "SST") |  | Contains separating gel and clot activator | 6.0 mL | Most chemistry, endocrine and serology tests, including Hepatitis and HIV |
| Light Blue - Yellow Label on Tube |  | Thrombin | 2.0 mL | For FDP test ONLY; Obtain tube from Core Lab Coag; Allow to Clot |
| Green |  | Sodium heparin(100 USP Units) | 5.0 mL | Ammonia, Lactate, HLA Typing |
| Tan |  | K2 EDTA | 5.0 mL | Lead levels |
| Yellow |  | ACD Solution A consists of trisodium citrate, citric acid and dextrose | 8.5 mL | DNA Studies, HIV Cultures |
| Pink |  | (K2)EDTA | Draws 6 mL - Minimum 4 mL | Blood type & Screen, Compatibility Study, Direct Coombs HIV Viral Load |
| Pearl Top (Plasma Preparation, "PPT") |  | Separating gel and (K2)EDTA | 4.0 mL | Adenovirus PCR Toxoplasma PCR HHV-6 PCR |
| Lavender ("Purple") |  | (K2)EDTA | 3.0 mL | CBC/Diff/Retic/Sed Rate, FK506, Cyclosporin, Platelet Ab, Coombs, Flow Cytometry |
NOTE: All tubes sterile.
Standard order of draw: BLOOD CULTURES, royal blue, red, light blue, SST (Gold), green, tan, yellow, pink, pearl, lavender. If a coag tube (light blue) is the only tube or the first tube to be drawn, a 5 mL discard tube must be drawn first.
Blood Culture Options (Specify Culture Type when Ordering)
| Culture Type | Patient Population | Test ID | Test # | BottleType/Comments | |
|---|---|---|---|---|---|
| Culture, Blood, Routine | All Patients not Meeting Criteria Below NOTE: This culture will recover HACEK organisms with no change in collection or incubation conditions. If Brucella or Francisella are in the differential, extended incubation may be required. Please contact the Microbiology Lab (966-4056). | BROU | 9500 |
BD BACTEC Plus Aerobic/F (Grey Cap/Blue Ring) (Optimal Volume 8-10 mL) Lawson# 052346(UOM box of 50 bottles) BD BACTEC Anaerobic Lytic/10 (Purple Cap/Maroon Ring) (Optimal Volume 8-10 mL) Lawson# 052347 (UOM box of 50 bottles) | |
| Culture, Blood, AFB | HIV+ patients with CD4 Count <100; Suspected MAC infections | AFBB | 9684 |
BD BACTEC Myco/F Lytic (White Cap/Red Ring) Available from Phlebotomy Lab (Optimal Volume 1-5 mL) | |
| Culture, Blood, Pediatric | Patients Weighing <40 kg | BPED | 9548 |  BD BACTEC Plus aerobic/F (Grey Cap/Blue Ring) (Optimal Bolume 8-10 mL) For Patients <20 Kg, 1-4 mL is acceptable For Patients 20-40 Kg, 4-8 mL in one bottle. For Patients >40 Kg (See Routine above) | |
| Blood Culture Mould | Suspected infections due to dimorphic (endemic) fungi, Fusarium sp., Trichosporon sp., Malassezia furfur (Specify at Collection). | BPATH | 9507 | Isolator Yellow Top (10 mL Volume) Available from Plebotomy BD BACTEC Plus Aerobic/F (Grey Cap/Blue Ring) (Optimal Volume 8-10 mL) Lawson# 052346 (UOM box of 50 bottles) BD BACTEC Myco/F Lytic (White Cap/Red Ring) Available from Phlebotomy Lab (Optimal Volume 1-5 mL) |
Source: https://www.uncmedicalcenter.org/mclendon-clinical-laboratories/test-specifications/tube-guide/
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